Description
Isocitrate dehydrogenase 1 (IDH1) is a 46 kDa NADP-dependent enzyme, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate. IDH1 may also play a role in the prevention of oxidative damage, and the shuttling of proteins to peroxisomes. It is widely reported that mutations in IDH1 result in multiple forms of gliomas. The Arginine to Serine substitution at amino acid 132 is seen in gliomas, abolishes magnesium binding and alters enzyme activity so that isocitrate is no longer converted to alphaketoglutarate but instead alpha-ketoglutarate is converted to R(-)-2- hydroxyglutarate.
Additional information
| Clone | SMab-1 |
|---|---|
| Isotype | IgG1k |
| Immunogen | KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1)-R132S |
| Species | Mouse |
| Cellular Localization | Cytoplasmic/nuclear |
| Positive Control Tissue | Human Glioma tissue |
| Pretreatment | Tris/EDTA Buffer pH 9.0 |
| Incubation & Temperature | 30 min @ RT |
| Intended Use | RUO |
| Detection System | PolyVue Plus – Two Step Detection System or Montage PolyVue Plus Auto Detection System for Montage 360 System |
| Description/Type | Mouse Monoclonal Antibody |
| Format | Tissue Culture Supernatant |
DATASHEETS & SDS
DATASHEETS & SDS
| Download R Datasheet |
| Download SDS Sheet – OSHA |
REFERENCES
REFERENCES
Kaneko, Mika Kato, et al. Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation.Biochem. Biophys. Res. Commun., 406: 608-13 (2011) 2011
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