40X Immuno Wash Buffer washes and removes excess reagents during immunostaining. It works well for manual and automated immunohistochemistry applications as well as other laboratory procedures requiring a high quality TBS buffer with superior pH stability. The TBS contains surfactant and allows for reagents to spread more uniformly across tissue section on slides.
Optimal immunostaining not only depends on the specificity of the primary antibody and other immunoreagents but also depends on obtaining a good signal to noise ratio. Binding of an antibody to its epitopes involves van der Waals forces, electrostatic forces and hydrophobic forces. Certain antibodies have tendency to bind loosely and nonspecifically to unrelated epitopes, which can create undesired background staining. In order to remove these nonspecifically bound antibodies, a thorough washing is required after each immunostaining step. Immuno Wash Buffer is specifically designed to remove such loosely bound antibodies effectively and efficiently and to provide a cleaner background staining.