This antibody recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. Its epitope is located between aa112-412. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin’s disease, CD30/Ki-1 antigen is expressed by mononuclearHodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).
BALB/C mice were immunized with L428 cell line.
|Positive Control Tissue
Hodgkins Lymphoma, Tonsil
EDTA Buffer pH 8.0
|Incubation & Temperature
30 min @ RT
PolyVue Plus – Two Step Detection System or Montage PolyVue Plus Auto Detection System for Montage 360 Systemrn
Mouse Monoclonal Antibody
This product is supplied as a purified immunoglobulin and contains sodium azide as a preservative.
- Schwarting R, Gerdes J, Durkop H, Falini B, Pileri S, Stein H. BerH2: A new anti-Ki-1 (CD30) monoclonal antibody directed at a formol-resistant epitope. Blood 1989;74:1678-89.
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