DAPI

DAPI or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells.

Features
• This DAPI solution is optimized for use on formalin-fixed, paraffin-embedded tissue sections.
• The DAPI solution contains an anti-freeze agent so the reagent can be stored at -20 °C without freezing.
• For convenience the DAPI solution can be removed from the freezer and used immediately without the necessity of thawing.
• There is no need to aliquot and freeze small portions.

Known Applications
• Fluorescence In Situ Hybridization
• Immunofluorescence with fluorescently labeled antibodies or probes.
• Fluorescent staining methods.

Product Description
This product is provided ready-to-use, and no further dilution is required. This product should be stored at -20 °C. The product contains an anti-freeze agent, so it can be used immediately after removal from storage and should be returned to storage immediately after use. When stored at -20°C, the product remains stable up to the expiration date indicated on the label.

DATASHEETS & SDS

REFERENCES

• Kapuscinski, J. (September 1995). “DAPI: a DNA-specific fluorescent probe”. Biotech. Histochem. 70 (5): 220–233.
• Scott Prahl, DAPI. accessed 2009-12-08