Traditional formulations for red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic for use in automated slide strainers. PermaRed/AP-AutoPlus has been formulated for on-slide and onboard mixing for any autostainer. Both components are stable and can be loaded at the beginning of the staining run, for walk away convenience.
PermaRed/AP-AutoPlus produces a red color, ranging from pink to brilliant dark red. It is insoluble in organic solvents; therefore sections can be dehydrated in alcohol, cleared in xylene (or a xylene-substitute), and permanently mounted. This chromogen substrate system may be used for both automation and manual use.
PermaRed/AP-AutoPlus is a substrate/chromogen system for use with alkaline phosphatase (AP) detection in immunohistochemistry (IHC) or in situ hybridization (ISH) run on automated staining instruments as well as manual use.