Phosphohistone H3 (PHH3)

Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis.Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis.PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST.

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REFERENCES

• Gurley LR, et al. Histone phosphorylation and chromatin structure during mitosis in Chinese hamster cells. A Eur J Biochem 1978; 84:1- 15
• Hendzel MJ, et al. Chromatin condensation is not associated ith apoptosis. J Biol Chem 1998; 273:24470-8
• Colman H, et al. Assessment and prognostic significance of mitotic index using the mitosismarker phospho-histone H3 in low and intermediate-gradde infiltrating astrocytomas. Am J Surg PathoL 2006; 30:657-64