Description
Antihuman isocitrate dehydrogenase 1 (IDH1) R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.
Additional information
| Clone | HMab-1 |
|---|---|
| Isotype | IgG1k |
| Immunogen | KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1) |
| Species | Mouse |
| Cellular Localization | Cytoplasmic/nuclear |
| Positive Control Tissue | Human Glioma tissue |
| Pretreatment | Tris/EDTA Buffer pH 9.0 |
| Incubation & Temperature | 30 min @ RT |
| Intended Use | RUO |
| Detection System | PolyVue Plus – Two Step Detection System or Montage PolyVue Plus Auto Detection System for Montage 360 System |
| Description/Type | Mouse Monoclonal Antibody |
| Format | This product is supplied as a purified tissue culture supernatant and contains sodium azide as a preservative. |
DATASHEETS & SDS
DATASHEETS & SDS
| Download R Datasheet |
| Download SDS Sheet – OSHA |
REFERENCES
REFERENCES
- Capper D, et al: Characterization of R132H mutation-specific IDH1 antibody binding in brain tumorsBarin Pathol 2010 Jan;20(1):245-254
- Capper D, et al: Application of mutant IDH1 antibody to differentiate diffuse glioma form nonneoplastic central nervous system lesions and therapy-induced changes. Am J Surg Pathol 2010 Aug;34(8):1199-1204
- Camelo-Piragua S, et al: Mutant IDH1-specific immunohistochemistry distinguishes diffuse astrocytoma form astrocytosis. Acta Neuropathol 2010 Apr;119(4):509-511
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